#LIOS-002
SUMMARY
Preclinical pharmacokinetic and safety studies in rodents assess ADME, bioavailability, and clearance to guide dosing. Using routes like oral, IV, or minipumps, and advanced analytics, the service identifies toxic effects, target organs, and supports safe, effective drug development.
BACKGROUND Pharmacokinetics (PK) help determine a compound’s bioavailability and clearance, guiding dosing strategies by tracking drug exposure over time. PK studies assess absorption, distribution, metabolism, and excretion (ADME) in mice and rats by measuring drug levels in plasma, tissues, or urine at set intervals. In safety studies, we identify target organs and monitor adverse effects from single or repeated dosing. Single-dose studies define the maximum tolerated dose, while repeated-dose studies reveal cumulative toxicity and potential organ damage.
FEATURES AND KEY BENEFITS
- Peroral, subcutaneous, and intravenous routes of administration;
- Slow-release minipumps, direct intracerebroventricular injections and other specific methods can be used for the administration of compounds.
- Analytics by LC-MS, GC-MS, or using fluorescent, luminescent measurements as well as scintillation counting. Metabolite identification can be performed using HRMS.
- Comprehensive overview of the compound’s safety profile by examining immediate and long-term toxicological effects.
- General safety testing and advanced methods for to specifically study manifestations of nephrotoxicity, cardiotoxicity, liver and CNS toxicity.
REFERENCES
[1] Brem, J., et al. Imitation of β-lactam binding enables broad-spectrum metallo-β-lactamase inhibitors. Nat. Chem. 2022; 14, 15–24 (). https://doi.org/10.1038/s41557-021-00831-x
[2] Becker K, et al. Antibacterial activity of apramycin at acidic pH warrants wide therapeutic window in the treatment of complicated urinary tract infections and acute pyelonephritis. EBioMedicine. 2021; 73:103652. https://doi.org/10.1016/j.ebiom.2021.103652
AVAILABLE
for service or research collaboration.
CONTACT
Dr. Anna Stikāne: anna.stikane@osi.lv
Latvian Institute of Organic Synthesis, Aizkraukles Str. 21, Riga LV-1006, Latvia
