#UZSM-002
SUMMARY
Cytotoxicity testing of new chemical entities and biological extracts against various cell lines.
BACKGROUND
Cytotoxicity testing is used in drug discovery projects to evaluate the safety profile of new substances. Cytotoxicity testing ensures determination of inhibitory concentrations of novel chemical entities and biological extracts, IC50, that inhibit the growth of cells in a culture.
FEATURES AND KEY BENEFITS
In vitro cytotoxicity screening incudes MTS cell viability assay which measures metabolic capacity of cells in culture. Substances are tested in microtiter plates, in serial dilutions using standard optimized media. Cell lines used include HepG2 and THP-1.
REFERENCES
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65(1-2): 55-63.
AVAILABLE
for service, contract research collaboration
CONTACT
Mihaela Peric, Centre for Translational and Clinical Research, University of Zagreb School of Medicine, Šalata 2, 10000 Zagreb, Croatia; mihaela.peric@mef.hr
