#ULMF-001
SUMMARY
The collection includes primary skeletal muscle cells (HSMCs) obtained from skeletal muscle tissue (m. semitendinosus) obtained as surgical waste during standard anterior cruciate ligament (ACL) reconstruction in subjects with ACL rupture. No tissue was harvested solely for research purposes.
BACKGROUND
Primary HSMCs (myoblasts and/or myotubes) are used as an in vitro model for the study of skeletal muscle physiology (e.g. myogenesis, muscle repair, contraction and metabolism), skeletal muscle pathology and regeneration as well as therapeutic interventions on skeletal muscle. Primary HSMCs allow researchers to study cellular responses to various factors, e.g. nutrients, hormones, and drugs, under controlled conditions. Myotubes can be stimulated to contract by electrical pulse stimulation or innervation. Informed patient consent and ethical approvals for the collection and use of HSMCs for the above purposes were obtained.
FEATURES AND KEY BENEFITS
Primary HSMCs offer several advantages over other skeletal muscle cell models, such as L6 rat skeletal muscle cells and C2C12 mouse skeletal muscle cells: 1) primary HSMCs are of human origin, making them a more relevant cell model for human skeletal muscle than rat L6 cells and mouse C2C12 cells; 2) primary HSMCs can have similar metabolic properties to donor skeletal muscle, allowing researchers to study the underlying causes of metabolic changes in skeletal muscle cells in vitro; 3) when using primary HSMCs from multiple donors, researchers can gain insight into the inherent variability between individuals.
REFERENCES
Jan V., Mis K., Nikolic N., Dolinar K., Petric M., Bone A., Thoresen G. H., Rustan A. C., Mars T., Chibalin A. V. and Pirkmajer S. (2021): Effect of differentiation, de novo innervation, and electrical pulse stimulation on mRNA and protein expression of Na+,K+-ATPase, FXYD1, and FXYD5 in cultured human skeletal muscle cells. PloS One, 16(2), e0247377, https://doi.org/10.1371/journal.pone.0247377.
Marš T, Miš K, Meznarič M, Prpar Mihevc S, Jan V, Haugen F, Rogelj B, Rustan AC, Thoresen GH, Pirkmajer S, Nikolić N. Innervation and electrical pulse stimulation – in vitro effects on human skeletal muscle cells. Appl Physiol Nutr Metab. 2021;46(4):299-308. Innervation and electrical pulse stimulation — in vitro effects on human skeletal muscle cells
AVAILABLE
for research collaboration
CONTACT
University of Ljubljana, Faculty of Medicine, Vrazov trg 2, 1000 Ljubljana, Slovenia, sergej.pirkmajer@mf.uni-lj.si
